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Afterwards, the animals were randomly divided into four experimental groups ( n = 6 per group): a- Reference group (RD) received the standard commercial rodent diet, b- HSD group received a home-made semisynthetic diet, based on AIN-93 M (23) with modifications, containing -g/kg diet-: sucrose 630, protein (vitamin-free casein, 85% protein) 200, fat (corn oil) 60, fiber (cellulose) 59.5, methionine 3.0, choline bitartrate 2.5, mixture of salts (AIN-93 M-MX) 35, mix of vitamins (AIN-93 M-VX) 10, c- RD + ASTX group received the standard commercial rodent diet plus ASTX, d- HSD + ASTX group received a HSD plus ASTX